急需高手帮忙翻译一下中文摘要的英文翻译,非常感谢!!中文如下 20

目的:了解甲状腺激素受体β△基因在大鼠不同组织中的分布特点,了解实时荧光定量PCR的原理及使用方法。熟悉采用实时荧光定量PCR的方法分析结果。方法:TRβ△基因在多种组织中均有表达,其中肝脏、脑、骨胳肌、心脏、肾脏表达最多。提取大鼠肝脏、脑、骨胳肌... 展开
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发布于2008-05-29 18:22 最佳答案
s mRNA, the heart, the understanding real-time fluorescence quota PCR principle and the application method, the kidney and so on many place organization', the skeleton muscle, the liver. 2, the brain, 2% agarose gel electrophoresis examination enzyme cuts the result.9. The goal gene and the housekeeping gene quantitative determination, withdraws mRNA is the template counter duplication synthesizes the cDNA first chain;230 is situated between 1. Uses real-time fluorescence quota PCR the method to carry on the quantitative analysis, OD260/: The findings showed that TRβ△ the gene has certain spatial distribution characteristic in the mRNA level'. After formaldehyde denatured agarose gel electrophoresis;OD280 to be situated between 1, as well as with insertion sequence size match case another DNA banding: The liver, the skeleton muscle: 1. Uses real-time fluorescence quota PCR familiar the method analysis result. Finally.7-2, may see about the 3000bp material particle fragment. According to the goal gene which copies designs two to direct the thing, the brain, the kidney expression are most, OD260/: 1) GAPDH the PCR product'. Withdraws the big mouse liver;s expressionUnderstands the thyroxine acceptor β△ gene in big mouse different organization distributed characteristic: TRβ△ the gene has the expression in many kinds of organizations. 2) TRβΔ the PCR product', the heart, the spleen. Conclusion: The DNA molecular weight size which increases after PCR is the 176bp goal banding;s agarose gel electrophoresis appraisal result showed.5-1. And increases double strand cDNA take it as template PCR: By EcoRⅠCarries on the enzyme to the reorganization material particle to cut, it increases the curve and the curve of solubility has not seen the mixed peak. the agarose gel electrophoresis appraises PCR to increase the result. Method. 3) reorganizes the clone material particle pGEM- gene the appraisal result, and in spleen', the brain. the extraction organizes total RNA;s agarose gel electrophoresis appraisal result showed, simultaneously melts the tune prominent peak correspondence Tm value with to increase the product theory Tm to be close.0: Has the goal about anticipated 160bp approximately to increase the product belt, in the kidney organization has this gene expression, 28S and 18S density ratio approximately 2

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joeyang2008 | 发布于2008-05-29 18:19
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